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Bicine is an organic compound used as a buffering agent. It is one of Good's buffers and has a pKa of 8.35 at 20 °C.

It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0.

It is prepared by the reaction of glycine with ethylene oxide, followed by hydrolysis of the resultant lactone.

Applications

Bicine is a contaminant in amine systems used for gas sweetening. It is formed by amine degradation in the presence of O2, SO2, H2S or Thiosulfate.

It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, Bicine is commonly used as a buffer in the polymerase chain reaction and for the extraction of enzymes from various plant species.