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BCA is a colorimetric reagent for the detection of copper (Cu+).

BCA and Cu+ form a stable 2:1 complex that has an absorbance maximum at 562 nm.1 It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu2+ is converted to Cu+ as a function of protein concentration under alkaline conditions.

Applications

BCA may be used as ligand in the aerobic oxidation of alcohols (primary, secondary and benzylic) in the presence of palladium catalysts.

BCA is useful for the determination of copper.

It is also used for the determination of protein concentration.

It is a biocompatible polymer that has been shown to have inhibitory properties in response to atherosclerotic lesions.

It is used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.

It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).

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