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CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid.

Applications

CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons.

CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral.

CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.

Tips

When using CHAPS to prepare any solution, please try not to shake or stir fiercely