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Features and Application of MOPS
Buffering Features
MOPS is a buffer introduced by Good et al. in the 1960s. Its chemical structure contains a morpholine ring.
With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers.
Applications
MOPS is a buffering agent used in many biological and biochemical applications. This free acid buffer is useful in maintaining pH levels and enzymatic activities.
MOPS is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels.
MOPS is used for Northern Blots -the hybridization of total RNA or mRNA to a membrane.
In the separation of nucleic acids and proteins by electrophoresis
MOPS is used as a buffer component of charcoal yeast extract medium
Tips
Usage above 20 mM in mammalian cell culture work is not recommended
MOPS can modify lipid interactions
MOPS are partially degraded by autoclave in the presence of glucose
MOPS can be oxidized by hydrogen peroxide, but the oxidation process is very slow
Mops interferes with Lowry protein determination