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Buffering Features

The conjugate acid of tris has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.1 and 9.1 (pKa ± 1) at room temperature.

Buffer details

• In general, as temperature decreases from 25 °C to 5 °C the pH of a tris buffer will increase an average of 0.03 units per degree. As temperature rises from 25 °C to 37 °C, the pH of a tris buffer will decrease an average of 0.025 units per degree.

• In general, a 10-fold increase in tris buffer concentration will lead to a 0.05 unit increase in pH and vice versa.

• Silver-containing single-junction pH electrodes (e.g., silver chloride electrodes) are incompatible with tris since an Ag-tris precipitate forms which clogs the junction. Double-junction electrodes are resistant to this problem, and non-silver containing electrodes are immune.

Applications

Tris is used for biochemical, microbiological, pharmaceutical and molecular biology purposes as a buffer substance or as a component of buffer solutions.

TRIS is considered an excellent pH adjuster, It has the feature of acidity regulators and maintains the activity of ingredients. TRIS can maintain the pH value of the product at pH4.75~pH5.75 to suit the human skin.

Take TRIS as synthetic skincare and cosmetic ingredients, is the common formulas on the cosmetics market, such as an emulsifying agent, ingredient thickener, and humectant. It also can be a fragrance that reduces or inhibits the taste of the product. Therefore, TRIS is widely used in sunscreen, leave-on nail creams, eyes makeup up, and lotions, and is known for its capacity to maintain the original colors of lotions after opening.

Tris is used as alternative to sodium bicarbonate in the treatment of metabolic acidosis.

For the evaluation of bacterial endotoxins.

For anion exchange chromatography

Tips

It is not very efficient below pH 7.54

Tris buffers need to be prepared at the right temperature

It is toxic for many mammalian cells

It has a high solubility in organic solvents

Tris also inhibits enzyme activity through chelation of metal ions