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Application of NBT
NBT, Product Name "Nitro Blue Tetrazolium Chloride", CAS is 298-83-9, is a compound composed of two tetrazolium groups. The following are their product uses:
1. NBT is the substrate of dehydrogenase and other peroxidase. It is used for the determination of glucose-6-phosphate dehydrogenase and also for the precipitation of coagulation factor.
2. It is also one of the substrates of alkaline phosphatase, and produces insoluble Blue products under the catalysis of alkaline phosphatase. It is commonly used in IHC, ICC and WB tests.
3. NBT can be used as a method to screen urinary tract infection. The serum glycosylated low density lipoprotein and very low density lipoprotein (SG lp-vp) were determined by NBT reduction method. The concentration of SG lp-vp was directly expressed by light density according to the color depth, and the index of SG lp-vp was calculated. The result is very good.