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Introduction and application of IPTG

Update Time:2020/3/20 15:36:10   View times:
 

Isopropyl β-D-1-Thiogalactopyranoside, abbreviated as "IPTG" in English, is a kind of chemical substance, white crystalline powder; soluble in water, methanol, ethanol, acetone, chloroform and ether. Here is the company's product information:

 

Product ID :TQDR019

Product Name: Isopropyl β - d-1-thiogalactopyranoside; IPTG

CAS NO:367-93-1

Molecular Formula:C9H18O5S

Molecular Weight:238.30
       Purity:≥99%
       Grade:Ultra Pure Grade
       Appearance:White Crystalline powder
       Application:IPTG is used in cloning experiments that require the induction of beta-galactosidase activity. It is often used in conjunction with the x-gal or bluo-gal to reorganize the blue-white screening of bacterial colonies that can induce the expression of lac manipulators in e. coli. IPTG plays a role in binding and altering the conformation of lacI repressors to prevent the inhibition of the beta-galactosidase encoding gene lacZ.


 

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