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Application of CHAPS

Update Time:2020/3/16 13:28:58   View times:

CHAPS is a non - transgendered ionic detergent used in membrane biochemistry.

It can be used to dissolve membrane proteins and disrupt protein-protein interactions. CHAPS has low micellar molecular weight (6,150) and high critical micelle concentration (6-10mm), so it can be removed from the sample by dialysis. It is also suitable for use in isoelectric focusing and two-dimensional electrophoresis to dissolve proteins. CHAPS is often used in non-transsexual (non-urea) isoelectric focusing, and it has been found that it has excellent resolution for certain subcellular preparation and plant proteins. The concentration is usually between 2 and 4% (w/v) for isoelectric focusing gel.

Storage Conditions:2-8°C


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