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Common methods of Guanidine Thiocyanate

Update Time:2020/3/11 11:29:38   View times:

Guanidine Thiocyanate (GITC), CAS No: 593-84-0. It is a kind of super affinity reagent, commonly used in protein denaturant, but most commonly used in DNA and RNA extraction.

The common method is thiocyanate phenol chloroform extraction. If RNA is extracted for Northern blotting or DNA is used for Southern blot analysis, it is not necessary to strictly use phenol or chloroform because the gel is transferred to the membrane after gel electrophoresis and RNA/DNA is separated from the protein. In addition, because these methods use probes to bind to their conjugates, peptides that pass through this process are generally not important unless the peptide is RNase or DNase, and only when the enzyme tries to regenerate, which should not happen if appropriate protocols are followed. One possible exception may be when dealing with temperature extremes, where some of their enzymes can remain stable under special circumstances.

Guanidine Thiocyanate can also be used for the lysis of cells and virus particles in RNA and DNA extraction. In addition to its function of cracking, it can also prevent their activity by denaturing RNA enzyme and DNase enzyme. Otherwise, these enzymes will destroy the extract.

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